Resumo. The knowledge that antioxidants play in inhibiting the free radicals resulting from cellular metabolism,has stimulated interest in the analysis of these compounds in various food products. The aim of this studywas to quantify the total phenolic compounds and its correlation with antioxidant activity, to determinethe antioxidant properties of honey produced by Apis mellifera L. the Pantanal. Were examined twentyfive samples of honey multiflora, collected in the Pantanal of Mato Grosso do Sul, only two experimentalfarms (Nhumirim and Band’Alta) with different geographical characteristics. The average levels of totalphenolics and antioxidant activity of honey farm Nhumirim were significantly higher (p<0.05) to thosefound in farm Band’Alta, with average values of 222.03 and 61.52 mg/100g and activity of 223,86 and120,93 μMTrolox, respectively. There seems to be some relation between the total phenolic content andantioxidant activity revealed, as the average value of the sample which is richer in this compound has alsopresents the average of the highest antioxidant activity. However, the results demonstrate that the activitystudied can not be predicted in view, only the parameter considered.Keywords: