Resumo. Abstract Aim The objective of this work is to record the occurrence of Apistogrammoides pucallpaensis in Brazilian territory, since so far its distribution is restricted to Peru and Colombia. Methods The collections were carried out in the year of 2013, during the research of ichthyofauna of the region of Auati-Paraná, located in the Mamirauá Reserve in the state of Amazonas. In the floating aquatic vegetation was used net and on the coastal region rapichés. Apistogrammoides is the only one among Geophaginae that has 6-9 spines in the anal fin, while Apistogramma has 3-4. Results Note the presence of three evident mark on the caudal fin and presence of a black stripe on the side of the body that follows from the eye to the caudal peduncle feature thet differentiate the genre. Conclusions This work extends the distribution of A. pucallpaensis being the first record of the species for Brazil.