Resumo. The objective of this study was to evaluate three systems of seedling production of interspecific hybrids of American oil palm and African oil palm (HIE), difference between 12 sib progenies of BRS Manicoré cultivar and the interaction between these two factors. The production systems were evaluated: (i) two- stage nursery with the initial shading (conventional system), (ii) two-stage nursery without shading and (iii) one-stage nursery without shading. The variables evaluated were plant height, number of leaves and stem diameter at 106, 191 and 393 days after sowing. In all variables and periods analyzed, the genotype and environmental factors were significant to F test, but the interaction genotype x environment was not significant. The best development of seedlings in the nursery production system occurred in one-step nursery without shading. The two stages nursery with shading in pre-nursery, which is currently the most widely adopted, resulted in lower seedling development.