Resumo. The current study aimed to identify pathogens, analyzing different seed treatments and substrates for the germination of Cabralea canjerana and also to indicate instructions testing for laboratory test of the species germination. First, we assessed the moisture content and determined the thousand seed weight. In order to assess the pathogens associated with the seeds, health testing was performed at different seed treatments: T1 - Control; T2-Captan®; T3-Maxim®; T4-Sodium hypochlorite; and T5-Agrotrich Plus®. The germination test was performed based on a factorial design with five seed treatment sand three substrates (on blotting paper, vermiculite and sand). The seeds showed moisture content and thousand seed weight of 41.2% and 457.3 g, Fusarium spp., Cladosporium spp., Pestalotia spp. and Phomopsis spp.. The seed treatment with Captan®, Maxim® and sodium hypochlorite was effective in reducing the incidence of Penicillium spp., but only the last two showed the greatest potential forseed germination, regardless the used substrate. Treatment with Agrotrich Plus® had the lowest percentage of germination and increased incidence of Fusarium spp. Asepsis with 1% per 2’ sodium hypochlorite and seeding substrate over vermiculite is indicated for the germination tests of Cabralea canjerana seeds and the first counting can be performed at the 19th day and the final one at the 30th day after the test.