Abstract. In recent decades, the human activities caused the overall decline in biodiversity and, to understand the impacts on plant communities, it is necessary to analyze the regenerative potential of the forest. Studies on the floristic composition and the structure of the guild of seedlings in different succession stages and historical conditions can describe the patterns of species replacement and help understand the forest dynamics. Thus, the objective of this study was to compare floristic and structural layer of the regeneration and associated vegetation in two succession stages of two areas with different historical uses located in the city of Campina Grande do Sul, in Paraná state. Seedlings were collected in two fragments of the secondary forest at the initial and at the intermediate stages, with historical use of clear and selective cuts, respectively. One-hundred and six plots of 0,16 m² were delimited and all individual plants with up to 10 - 50 cm high were collected, identified, quantified and divided into two groups: seedlings of tree species and species associated for the phytosociological analysis. The species were also classified according to their habits, dispersion mode and succession stages and the floristic similarity between areas was established by Sorensen's, Bray & Curtis' and Morisita-Horn's indices. The analysis suggests a possible influence of historical use and the successional stages of regeneration and the associated vegetation. The analysis of similarity indicates a gradient of regeneration, in which the intermediate stage of the clear cut area is similar to the early stage of the selective cutting area.