Resumo. The purpose of this work was to evaluate the preservative treatment effect and the adhesive type in physical-mechanical properties of glue laminated bamboo (GLB) of Dendrocalamus giganteus. Thus, the bamboo stems were treated by immersion method with chemical product (solution of 1% of active ingredients of copper chrome borate - CCB) and water by 15 days. Before treating the stems, the external and internal parts were removed to obtain the flat strips of bamboo with 4 mm of thickness, 45 cm of length and 5 cm of width. The "Cascophen RS" (resorcinol-formaldehyde) and "Cascorez-extra" (polyvinyl acetate) commercial adhesives were used to bond the flat strips. The resorcinol-formaldehyde adhesive showed superior moisture resistance than polyvinyl resin to GLB, and the samples obtained of pieces treated with CCB were more stable than treated with water. Among the mechanical properties tested, theadhesive factor was significant for the resistance to shear parallel to glue line and the preservative treatment for the rigidity. The interaction of the factors adhesive and treatment was significant for the rigidity, resistance to parallel compression and shear parallel to glue line. The pieces treated with water and adhered with resorcinolformaldehyde presented better results for the rigidity and parallel compression to the fibers and those treated with CCB, for the shear parallel to glue line.