Resumo. This paper aimed to evaluate the effect of leaf area reduction of apical and intermediate mini-cuttings and different substrates (vermiculite and organic substrate) on the rooting of six half-sib Anadenanthera macrocarpa progenies from mini-stumps of seminal origin. The evaluations were carried out in the greenhouse (30 days) and the shade house (40 days) for survival rate and roots percentage observed at the bottom of the container. Survival rate and rooting percentage, height, stem diameter, number of roots, and dry mass of shoot and roots of mini-cuttings were evaluated, in the full sunlight area, 70 days after staking. The results showed that are not differences between progenies for type of mini-cuttings and substrate to characteristics evaluated. The vermiculite substrate provided the best results for the survival rate, rooting percentage, height, stem diameter and weight of dry roots. In general terms, apical mini-cuttings up to 10 cm in length and entire leaf had higher averages survival rate, rooting percentage, root number and dry weight of shoot and root. Therefore, using vermiculite as substrate, apical mini-cuttings up to 10 cm in length and entire leaf were more appropriate for vegetative propagation from seminal material of Anadenanthera macrocarpa by mini-cutting technique.