Resumo. The production of native species seedlings with quality in order to achieve success in reforestation is related with the level of efficiency of used substrates. The objective of the study was to assess different substrate compositions for the production of seedlings in the city of Autazes, in Amazonas state. Eight treatments arranged in random blocks experimental design, with five replicates were tested. The treatments were: cattle manure, lime, corrective phosphate, fertilizer with NPK, lime + correction phosphate, corrective phosphate + fertilizer with NPK, lime + fertilization with NPK and lime + corrective phosphate + fertilizer with NPK. After 120 days, the following characteristics were assessed: height of shoot, diameter of the neck, shoot, root and total dry matter, nutrient levels and amounts in shoot dry matter. The results corroborate that all treatments provided improvements in the soil attributes. Those improvements caused larger readiness of nutrients for the plants, which provided an appropriate growth of Macacaúba seedlings. The plants that grew on cattle manure treatment showed higher nutrient absorption and growth rates in a smaller period of time. It suggests that cattle manure soil is the most suitable in the production of Macacaúba seedlings.