Resumo. The nutritional status of the matrix plant has a great importance for the maintenance of its vegetative vigor, which determines the sprouts production and also the rooting percentage and the speed of root formation, since the macro and micronutrients are involved in biochemical and physiological vital processes for the plant. Thus, this study aimed to evaluate the influence of NPK fertilizer on dry biomass production by minicuttings and mini-stumps and the percentage distribution of nutrients in a simple hybrid plant of Eucalyptus urophylla in nutrient solution. The experiment was realized from November, 2008 to January, 2009 in a vegetation environment. The experimental design was a completely randomized in factorial scheme with a fractional factorial (4x4x4)1/2, adding up to 32 treatments with three replicates. The treatments consisted of four levels nutrients of nitrogen (50, 100, 200 and 400 mg L-1) in the urea form, P (7.5, 15, 30 and 60 mg L-1) in the phosphoric acid form and K (50, 100, 200 and 400 mg L-1) in the potassium chloride form, in the nutrient solution. There was significant effect only to the N doses to the dry mass of mini-cuttings and the dry mass of shoots and roots of mini-stumps with decreasing linear effect against N doses increasing. The doses of 50 mg.L-1 of N promoted higher accumulation of dry mass in mini-cuttings and mini-stumps. The percentage distribution of dry mass and of the nutrients in dry mass of mini-stumps, shoots and roots of mini-stumps varied in relation to N doses. Macronutrients extraction from mini-stumps of 6 cm at a dose of 50 mg L-1 of N followed the order of decreasing N > S > P > K > Ca > Mg and micronutrients Cu > B > Mn > Fe >Zn.