Resumo. Setting priorities in the conservation of terrestrial ecosystems refers to the need for knowledge about the diversity of environments, especially in the Cerrado biome and its floristic transition zones, already in high stage of degradation. With this scenario, t his study aimed to carry out a floristic inventory of a forest fragment on ecological tension in Bodoquena Plateau, MS, Brazil, and assess their phytogeographical relationships with other surveys of seasonal forests and savannas in the Midwest and Southeast regions of Brazil. For this, 102 contiguous plots of 10x10 m (100 m 2 ) were sampled. The inclusion criterion was the perimeter at breast height (PBH) ≥ 15 cm. For floristic similarity analysis, the coefficient of Sorensen was applied, adopting the unweighted pair group method with arithmetic mean (UPGMA), with the determination of the level of similarity of 26 floristic lists. Of the 96 species occurring in this survey, 91 were identified at species level, and of these, 80.2% (73) were found in one or more studies of the analyzed areas with higher occurrence in seasonal deciduous forest (SDF) (54), followed by seasonal semideciduous forest (SSF) (47) and forested savannah (46). The results showed clustering of the study area with the FED of Minas Gerais in level of 30%, revealing low similarity. Due to low floristic similarity between the analyzed areas, these are characterized by their high beta diversity.