Resumo. Bark beetle contributes to the decomposition process of fallen or newly felled trees and are potentially harmful in forest environments, whether natural or planted. The objective of this work was to evaluate an assembly of bark beetle (Curculionidae: Scolytinae) in a planting of Eucalyptus urophylla x Eucalyptus grandis in Southern Amazonia, in the municipality of Alta Floresta, from August 2015 to August 2016. Twelve ethanol-trapped impact traps were used, and collections were performed biweekly. The collected insects were sent to the UNEMAT Laboratory, where they were sorted and sent to the Federal University of Paraná, for the correct identification of the species sampled. A total of 1,248 individuals were collected, with a total of 13 species in six genera, most representative of Cryptocarenus, Hypothenemus and Xyleborus. In the faunistic analysis Criptocarenus diadematus Eggers, Criptocarenus heveae (Hagedorni) and Criptocarenus seriatus Eggers occurred as dominant, very abundant, very frequent and constant, with aggregate distribution, being considered as potential insect pests in forests planted in the region.