Resumo. Diseases are spreading like a trend and victimising every other individual globally. Here, we are referring to one such most common disease that has not even spared young lives i.e., diabetes. Annually, several people lose their lives and loved ones because of this dangerous disease. This has compelled the researchers to think and work on some life saving treatment. People suffering from hyperglycaemic conditions have insulin resistance which can be improved by intake of myo-inositol. Myo-inositol has potential to regulate this insulin hormone which can prevent and control diabetes mellitus. In this research, we have used a natural source (fruit) Hylocereus undatus; it has proved to be a good source of myo-inositol and some proteins that help in insulin regulation naturally. Several techniques and tests were performed such as extraction, purification, crystallisation, proteolytic activity assay, protein estimation, etc. Positive results of myo-inositol were observed through crystallisation together with decent amount of protein concentrations by Folin Lowry test and SDS-PAGE analysis.