Resumo. Schubertia grandiflora is a species belonging to the floral of the Cerrado domain, presented annually with aromatic flowers. The study aimed to evaluate the hydroethanolic floral extract of S. grandiflora for phytochemistry, physicochemical and photoprotection, antioxidant, antifungal and antibacterial activities. Fresh flowers were collected and the floral extract produced by maceration. Qualitative phytochemical tests were carried out for the main phytochemical classes, organoleptic physico-chemical parameters, extract mass, moisture content, pH, relative density, refractive index, phenolic and total flavonoid contents, color density, Hue tint, and tests biological factors for protection factor, DPPH free radical reduction activities, antifungal and antibacterial. Phytochemistry has demonstrated the presence of several groups of important metabolites mainly in pharmaceutical use, visual color of the citrus yellow floral extract, aromatic and homogeneous, extract mass = 12.57%, moisture content = 6.21%, pH = 5.85, refractive index = 1.4217 nD, relative density 0.9044 g mL-1 20 °C, total phenolics = 238.83 mg GAE 100 g-1 dried extract, total flavonoids = 17.93 mg QC 100 g-1 dried extract, color density = 1.05, Hue color = 8.24, protection factor UVA and UVC, antioxidant activity with CI50 = 9.44 µg mL-1. Antifungal activity demonstrated inhibition zone only for Candida tropicalis between 10-6 mm and for Candida krusei between 18-5 mm, and antibacterial for Escherichia coli between 13-7 mm, Pseudomonas aeruginosa between 21-5 mm and Enterococcus faecalis between 32-17 mm. The hydroethanolic floral extract of Schubertia grandiflora demonstrated potential biological activities, characterizing this natural product for the development of pharmaceutical, biological and agricultural formulations.