Resumo. Propolis is a resinous product rich in phenolic compounds and flavonoids produced by bees. This study aimed to evaluate the propolis of Tetragonisca angustula regarding its classification group, ultraviolet and visible spectroscopy (UV-Vis) and its antifungal biological activity against Sclerotinia sclerotiorum, Colletotrichum gloeosporioides and Colletotrichum acutatum phytopathogens of grains and fruits of interest commercial. Propolis was collected from T. angustula hives in a permanent preservation area. The ethanolic extract was produced by static maceration. The classification group according to the dermal color, the determination of the bands were obtained in a UV-Vis spectrophotometer between 450-800 nm, the antifungal activity carried out in in vitro PDA medium at different concentrations on S. sclerotiorum, C. gloeosporioides and C. acutatum and expressed as a percentage (%). The group in which T. angustula propolis extract fits is the 12 SP12 group, UV-Vis bands identified the presence of flavonoids and phenolic compounds and the antifungal activity demonstrated that S. sclerotiorum is more sensitive when comparing the two species Colletotrichum fungi evaluated.