Resumo. Biodegradable films of arrowroot starch were incorporated with floral extract of Tabebuia impetiginosa and copper sulfate and determined to the physical, physicochemical, biodegradability and antibacterial properties. The films were produced from arrowroot starch with different concentrations of glycerol, floral extract and copper sulfate solution. Thickness, humidity, solubility, biodegradability, water vapor colorimetry, color density, transparency, FT-IR and antibacterial activity tests were performed for Escherichia coli, Staphylococcus aureus, Salmonella serovar Thyphymurium and serovar Enteritidis. The films had a thickness between 0.23-0.33 mm, humidity between 8.53-12.22%, biodegradability between 88.98-100%, water vapor between 255.79-433.57 g m2 day, L* between 4.77-22.36; a* between -1.20 to 0.30; b* between -1.89 to 0.13; color density between -0.09 to 0.180; maximum transparency of 80%, inhibition activity between 9.13-26.26 mm for E. coli, 5.83-24.14 mm for S. aureus, 13-42-19.39 mm for S. serovar Thyphymurium, and 22.14-26.89 mm for S. serovar Enteritidis. The arrowroot biodegradable films incorporated with floral extract of Tabebuia impetiginosa and copper sulfate showed good physical, physicochemical, biodegradability and potential antibacterial activity.