Resumo. We analyzed 614 specimens of Ichneumonidae (Hymenoptera: Ichneumonoidea) collected from a coffee agro-ecosystem located at Cravinhos, São Paulo, Brazil, and 34 nominal species were identified belonging to 22 genera and 10 subfamilies. Temelucha hilux Gauld, 2000 and Xiphosomella bonera Gauld, 2000 were recorded for the first time in South America, Colpotrochia diabella Gauld & Sithole, 2002 for the first time in Brazil, and Acerastes pertinax (Cresson, 1872), Colpotrochia mexicana (Cresson, 1868), Colpotrochia neblina Gauld & Sithole, 2002, Colpotrochia texana (Cresson, 1872), Diplazon mulleolus Dasch, 1964, Eiphosoma nigrovittatum Cresson, 1865, Enicospilus flavus (Fabricius, 1775), Enicospilus glabratus (Say, 1835), Enicospilus purgatus (Say, 1835), Lymeon haemorrhoidalis (Taschenberg, 1876), Mesostenus alvarengae Porter, 1973, Microcharops plaumanni Gupta, 1987, Nonnus niger (Brullé, 1846), Ophiogastrella maculithorax Brues, 1912, Pachysomoides stupidus (Cresson, 1874), Polycyrtus albolineatus Cameron, 1911, and Trieces horisme Gauld & Sithole, 2002 for the first time in the state of São Paulo. Other 14 species had been already registered for the state of São Paulo, and for the first time, were being recorded in relation to a coffee agro-ecosystem.