Resumo. ABSTRACT This study has evaluated the differences in the physiological and neuromuscular responses in female futsal athletes during a friendly game. Sample comprised six female futsal players (21.8 ± 3.9 years; 57.4 ± 4.1 kg; 159.0 ± 2.7 cm height; 19.0 ± 3.3% body fat and 7.8 ± 1.8 years of practice). Heart rate (HR) was monitored during the whole game. Coutermovement Jump (CMJ), Resistance Explosive Power (REP) lower limb and blood lactate have been evaluated before and after the final of the first and second periods. To compare physiological and neuromuscular variables between the different moments we have used standardized mean difference (SMD), confidence interval (CI = 90%) and the probabilities of differences with clinical relevance. Maximal heart rate (HRmax) was 186.6 ± 10.6 and 185.8 ± 8.3, first and second periods, respectively. There has been a substantial lactate increasing in the moment prior to the start of the game to the final of the first (2.1 ± 0.3 to 6.3 ± 2.3 mmol/L-1, SMD = 5.25 (3.28: 7.21), 100/0/0; most likely) and the second periods (2.1 ± 0.3 to 5.4 ± 1.3 mmol/L-1, SMD = 4.62 (3.19: 6.04), 100/0/0; most likely). Although there has been an increase in blood lactate, the performance of female futsal players was not suppressed during and immediately after a friendly game, suggesting that the neuromuscular system may be restored immediately after the end of the game.