Resumo. ABSTRACT The purpose of this study was to investigate the effect of game-based karate training on learning basic karate skills and karate children's interest in physical activity. The statistical population of this research included all 6 to 8-year-old female karate players in Sowmeh Sara city, Iran and twenty subjects were randomly selected and divided into two experimental and control groups of 10. The experimental group practiced game-based karate training for eight weeks. The training program was designed based on the principles of the Fundamental stage of the Canadian model of long-term development of athletes (LTAD) for karate sport. Physical activity enjoyment scale in children was used to measure children's interest in physical activity and karate yellow belt test was used for measurement of learning basic karate tasks. ANCOVA test was used for data analysis using SPSS 24 software at a significance level of 0.05. The results showed that there was no significant difference in the learning of basic karate skills, but a significant effect was seen in the enjoyment of children in the experimental group for physical activity (sig<0.001). Therefore, game-based karate training can help children learn basic karate skills as much as the traditional method, and also increase their enjoyment in physical activity more effectively.