Resumo. AIM: We present an update on the composition of the fish species of the Restinga de Jurubatiba National Park and the Imboassica Lagoon, in the northern portion of Rio de Janeiro State, based on collecting efforts of almost two decades, historical museum records, and a review of the literature. METHODS: Specimens were collected using a variety of techniques, mostly between 1994 and 2012, and were fixed with the use of a 10% solution of formalin and subsequently stored in 70% alcohol solution. All specimens examined are deposited in the fish collections of the Núcleo em Ecologia e Desenvolvimento Socioambiental de Macaé, Universidade Federal do Rio de Janeiro (NPM), and Museu Nacional, Universidade Federal do Rio de Janeiro (MNRJ). Continental (freshwater) fishes and some marine or estuarine species frequently collected were regarded as Resident, whereas marine species collected only sporadically were regarded as Occasional. Possible associations between the orientation of the lagoons and the composition of Resident fishes were explored through a Correspondence Analysis (CA). RESULTS: A total of 100 species, belonging to 19 orders and 41 families of the Teleostei were recorded. When both Resident and Occasional species are considered, families Carangidae and Engraulidae, with nine and eight species respectively, are the most representative. When only Resident species are considered, the Gerreidae and Gobiidae, both with seven species, are the most representative families. The Imboassica Lagoon, with 76 species, has the highest species richness among lagoons included in this study. A total of 81 species were recorded in the Restinga de Jurubatiba National Park, a number two times higher than reported on previous studies. An identification key, including 57 Resident species, is also presented. CONCLUSIONS: Parallel lagoons were characterized by a small group of continental species presumably of marine ancestry (Secondary Division), while marine species were more representative in Orthogonal lagoons. The higher frequency of marine species in Orthogonal lagoons is possibly associated with sandbar openings. Temporary and sporadic connections between Parallel lagoons during periods of higher rainfall may promote a higher similarity in terms of species composition.