Resumo. Aim: This study aimed to assess the spatial and seasonal variation of the water quality and physical habitat characteristics along the upper-middle stretch of the Paraitinga River, a tributary of Tietê River, considering the potential influence of different riparian conditions along the stretch studied.MethodsSixteen sites with different riparian vegetation, including native forest, secondary forest, pasture, and eucalyptus were sampled during the dry and rainy seasons of 2004/2005, before the damming of the Paraitinga Reservoir. Several physicochemical and habitat parameters were determined and data analyzed in relation to spatial distribution and potential influence of riparian conditions.ResultsWater quality parameters were in general within the limits established by CONAMA for Class 2 waters, except for turbidity and total phosphorus. There were seasonal and spatial differences in the limnological parameters along the stretch studied and apparently they were related to point specific influences associated with land use and canopy cover. Habitat characteristics were markedly different between the upper and middle river stretches, especially in relation to depth, width, substrate and canopy cover.ConclusionsAlthough a direct influence on the observed variables could not be attributed solely to the riparian vegetation, vegetation cover seemed to affect particular stream characteristics. Open pasture and eucalyptus sites were subject to point specific effects that caused phosphorus inputs and higher turbidity and temperature, and showed different morphological features, suggesting that land use at the sub-watershed scale was an important factor affecting stream conditions.