Resumo. The Cerrado and Atlantic Forest biomes are global biodiversity hotspots. However, these environments still face large knowledge gaps concerning fauna inventory studies, such as those on harvestmen. Our study aimed to sample harvestmen species in a transition area between the Atlantic Forest and Cerrado, contributing to future biodiversity assessments and conservation efforts in Brazil. The surveys were conducted in the municipality of Luminárias, southern Minas Gerais state, between May 2023 and March 2024. We collected 185 individuals from six species and three morphospecies with a wide geographical distribution, but whose conservation status has not been assessed, which may affect their protection. Additionally, loss of native vegetation may lead to reductions in their populations. Our study supports current literature, which suggests the need for more inventories and the creation of a Conservation Unit in the Luminárias region.