Resumo. The spatial pattern of regeneration of Aspidosperma polyneuron Müll.Arg. was studied in a remnant sector of the Paranaense forest in the Misiones province, Argentina. All individuals were mapped in a 210 m x 100 m area (2,1 ha). Univariate and bivariate point pattern analyses were used by means of Wiegand and Moloney O ring index. Regeneration showed clumped pattern to scales up to10 m and clusters of 30 m of diameter. No association was found between regeneration and both adult trees of A. polyneuron and standing death trees, whereas a small scale relationship between regeneration and adult trees of all species was observed. These results revealed that the regeneration of Aspidosperma polyneuron is strongly restricted to the presence of conspecific adult trees. On the other hand, the recruitments are associated with individuals from different species with DBH > 10 cm and infrequent in open sites.