Resumo. Eucalyptus globulus hybrids represent an excellent alternative to the pulp and paper sector, because of gains in wood quality for pulp. However, these hybrids have shown recalcitrance to adventitious rooting. Thus, micropropagation is considered the technique for rejuvenating adult genetic materials, allowing its clonal propagation. This study evaluated the in vitro culture of three clones of Eucalyptus grandis x Eucalyptus globulus and three clones of Eucalyptus urophylla x Eucalyptus globulus in relation to in vit ro multiplication on MS medium supplemented with 0.5 mgL -1 BAP and 0,01 mgL -1 NAA. The effect of IBA concentrations (0.25; 0.50; 0.75 and 1.0 mg L -1 ) and MS and JADS culture media was evaluated on in vitro elongation. The clones differed during in vitro multiplication of shoots and they had an average multiplication rate of 3.0 shoot clusters in each subculture. The responses of the clones to different concentrations of IBA, as well as to MS and JADS culture media differed for elongation in vitro . The range between 0.40 and 0.80 mg L -1 AIB provided the highest number and length of in vitro elongated shoots and the best vigor of shoots.